Lung (top section) Slice 1 Under the Microscope

Lung (top section) Slice 1, Homo sapiens

Lung (top section) Slice 1, prepared microscope slide

This is a frozen section of human lung tissue stained with H&E. It shows the tiny air sacs (alveoli) where oxygen passes into the blood, and it also shows the tears and folds that often happen when soft, airy tissue is frozen and cut.

This is a frozen section of human lung (Homo sapiens), stained with H&E. Much of the tissue is a lacy network of tiny air sacs called alveoli, where oxygen passes into the blood. The walls between the air sacs are very thin, so gases can cross them easily. Thick-walled blood vessels wind through the tissue, and some areas are deep pink to red from blood that has pooled in the tissue. Along the bottom edge, a dark purple band shows collapsed lung, where the air sacs have closed and the cells are pressed together.

What to look for

Alveoli: Many small, open, empty-looking spaces that give lung tissue a lacy, honeycomb look.
Alveolar walls (septa): Very thin pink walls between the air spaces, dotted with purple nuclei.
Blood vessels: Tubes of various sizes, some filled with bright pink to red blood cells.
Collapsed areas: Darker, denser patches where the air spaces have closed up, often near the edges.
Folds and tears: Darker creases and gaps caused by cutting frozen tissue, not by the lung itself.

Questions and answers

What does lung tissue look like under a microscope?

Lung tissue looks like lace or a sponge. Most of it is tiny air sacs called alveoli, separated by very thin walls. With H&E stain, the walls are pink with purple nuclei, and blood in the vessels is red.

Why are alveolar walls so thin?

Oxygen and carbon dioxide move between the air and the blood by diffusion, which only works well over very short distances. The thin walls put the blood in tiny capillaries very close to the air.

What is a frozen section?

A frozen section is made by freezing tissue and slicing it right away instead of embedding it in wax. It is fast, but soft or airy tissue like lung often tears or folds, so some of the gaps and creases on this slide come from the cutting process.

Compare it

How to identify tissue types

Based upon data generated by the TCGA Research Network (https://www.cancer.gov/tcga) and made available through the NCI Genomic Data Commons (GDC). See credits.

Category: Histology and Tissue
Scan magnification: 20x
Stain: H&E

Viewing this slide in class

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